Manual

Run Diagnostics and Details - Velocity Data:

Before experimental data can be analyzed, the data needs to be edited with the UltraScan editing module. In order to provide you with maximum convenience and user friendliness, UltraScan will automatically execute any functions which can be implemented without user intervention. These functions include certain diagnostics and analyses. To inform you of the results of these automated functions, UltraScan will display the Run Diagnostics/Run Detail window after an experimental data directory has been specified, or after an edited run has been loaded by a data analysis routine:

This window will inform you about all the salient features of a particular experiment and allow you to assign a unique run identification to the experiment that will be associated with all data analyses performed later on, as well as any result data files that will be derived from this data.

• Temperature Variation: This plot window shows you the temperature of each scan acquired during the experiment. The purpose of this plot is to inform you if a temperature variation during this run has occured that exceeds the temperature tolerance setting in the configuration file of UltraScan. It is important for velocity data that the temperature of the run does not vary over the course of the run for the following reason: A variation in temperature will affect the viscosity and density of the buffer/sample solution which cannot be compensated for in the data analysis. This will cause the S-value of the sample to vary during the course of the run, and most definitely introduce undesired inaccuracies in the results.

  Clicking on the "Time Plot" button will present you with a plot of the time differences between scans. The "Time Plot" button will then change to a "Speed Plot" button. Clicking on the "Speed Plot" button will then show you a plot of the speed at which each scan was recorded. Finally, you can recall the temperature plot by clicking on the "Temp. Plot" button, and cycle through the different plots by repeatedly clicking on the same button.

• Temperature Check: If the temperature check has been exceeded during the run, this LCD will show a flashing red light, otherwise, the LCD will display a constant green light.
• Help Button: clicking on this button will bring up this page.
• Cancel Button: After selecting the incorrect directory, or to abandon the analysis of the selected run, you can press the "Cancel" button to abort. You can also press this button to close the Details window if it was opened as part of loading an edited velocity dataset.
• Accept Button: After entering the Run Identification of the selected data, you can click on the "Accept" button to return to the main editing window. If you fail to enter a Run Identification, a reminder will pop up to prompt you to enter a Run Identification before proceeding. If this window is called from a data analysis window, the "Cancel" and "Accept" buttons simply close the window.
• Data Directory: This is the location where the experimental data acquired by the Beckman Data Acquisition program is located.
• Run Identification: This is an editable field that allows you to enter a unique run identification for your experiment. This identification will be associated with all data analyses performed later on, as well as any result data files that will be derived from this data. Before allowing you to proceed, UltraScan requires that this field has a valid entry. A valid entry is any alphanumeric character, except a space (" "). If you fail to enter a Run Identification, a reminder will pop up to prompt you to enter a Run Identification before proceeding. If this window is called from a data analysis window, this field can't be edited and simply will display the Run Identification that was entered during the editing process.
• Cells with Data: This field will indicate which cell position(s) in the rotor where acquired in the experiment. Valid numbers: 1-8. In velocity runs, cell #8 is reserved for the counterbalance.
• Avg. Temperature: The average temperature of the run resulting from all scans in the experiment.
• Time Correction: The time correction resulting from the rotor acceleration period. Subtracting this number from the time of the run will result in the theoretical time that would have been observed if it would have been possible to start the run at exactly the rotor speed setting from the experiment. During the acceleration period the rotor is not spinning at constant speed, so a time correction is a good way to correct for that. It is obtained by subtracting the time of the scan from the Omega-square-t value recorded in the scan file.
• Length of Run: The time point at which the last scan of the experiment was taken.
• Rotor Speed: The rotor speed setting of the experiment.
• Cell Description: This is the description of the cell contents that were entered at the time of data acquisition. This field is editable and you can update or change this information at this time. When this window is called from a data analysis window, any changes in this field will remain without effect.
• Cell Listbox: This box allows you to review the Cell Description, Wavelengths and Scans acqired for each cell. As you scroll through this box and click on individual cells, the information in the respective fields will be updated for you to review.
• Wavelengths: The wavelength selection for the data. For absorbance data, there are up to 3 different selections available.
• Scans: The number of scans for each wavelength acquired.