Manual

Run Diagnostics and Details - Equilibrium Data:

This window will inform you about all the salient features of a particular experiment and allow you to assign a unique run identification to the experiment that will be associated with all data analyses performed later on, as well as any result data files that will be derived from this data.

• Temperature Variation: This plot window shows you the temperature of each scan acquired during the experiment. The purpose of this plot is to inform you if a temperature variation during this run has occured that exceeds the temperature tolerance setting in the configuration file of UltraScan. For equilibrium data it is desirable to maintain a constant temperature throughout the run for the following reason: A variation in temperature will affect the viscosity and density of the buffer/sample solution which would require a different temperature correction for each scan in a global analysis, which may be tedious to accomplish in the data analysis software and may not be supported in every analysis software package. Although a variation of the viscosity during an equilibrium run is irrelevant for the the analysis of truly equilibrated scans, a change in density throughout the run will affect the data analysis results if the buffer conditions are not properly adjusted for each temperature change.
• Temperature Check: If the temperature check has been exceeded during the run, this LCD will show a flashing red light, otherwise, the LCD will display a constant green light.
• Help Button: clicking on this button will bring up this page.
• Cancel Button: After selecting the incorrect directory, or to abandon the analysis of the selected run, you can press the "Cancel" button to abort.
• Accept Button: After entering the Run Identification of the selected data, you can click on the "Accept" button to return to the main editing window. If you fail to enter a Run Identification, a reminder will pop up to prompt you to enter a Run Identification before proceeding. If this window is called from a data analysis window, the "Cancel" and "Accept" buttons simply close the window.
• Data Directory: This is the location where the experimental data acquired by the Beckman Data Acquisition program is located.
• Run Identification: This is an editable field that allows you to enter a unique run identification for your experiment. This identification will be associated with all data analyses performed later on, as well as any result data files that will be derived from this data. Before allowing you to proceed, UltraScan requires that this field has a valid entry. A valid entry is any alphanumeric character, except a space (" "). If you fail to enter a Run Identification, a reminder will pop up to prompt you to enter a Run Identification before proceeding. If this window is called from a data analysis window, this field can't be edited and simply will display the Run Identification that was entered during the editing process.
• Cells with Data: This field will indicate which cell position(s) in the rotor where acquired in the experiment. Valid numbers: 1-8 (in absorbance experiments cell #8 is reserved for the counterbalance).
• Avg. Temperature: The average temperature of the run resulting from all scans in the experiment.
• Length of Run: The time point at which the last scan of the experiment was taken.
• Available Rotor Speeds: The rotor speed setting for all scans in the method scan of the equilibrium experiment.
• Cell Description: This is the description of the cell contents that were entered at the time of data acquisition. This field is editable and you can update or change this information at this time. When this window is called from a data analysis window, any changes in this field will remain without effect.
• Cell Listbox: This box allows you to review the Cell Description, Wavelengths and Scans acquired for each cell. As you scroll through this box and click on individual cells, the information in the respective fields will be updated for you to review.
• Wavelengths: The wavelength selection for the data. For absorbance data, there are up to 3 different selections available.
• Scans: The number of scans for each wavelength acquired.