Manual

2-dimensional Spectrum Analysis Grid Control Module:

The 2-dimensional spectrum analysis grid control module is used to select the parameters needed in a grid-based analysis using the 2-dimensional spectrum analysis method. The method supports a global analysis of multiple datasets, and parameters selected here apply to all datasets included in the analysis. For example, an experiment of multiple components has been done at multiple speeds. Each speed can provide a dataset to the global analysis of this system.

Analysis Parameters:

Minimum/Maximum s-value:This controls the s-value range examined in the spectrum analysis. If distribution was loaded, these values will be will be automatically initialized to reasonable values, otherwise they will need to be manually adjusted by the user. The larger the range, the more points will be needed to cover a sufficiently narrow grid for the analysis. s-value Resolution: This value controls the number of divisions made in the s-value range. f/f0 Minimum/Maximum: This controls the frictional ratio range examined in the spectrum analysis. The default setting is to search a space between an f/f0 range between 1 and 4. The larger the range, the more points will be needed to cover a sufficiently narrow grid for the analysis. Globular proteins often can be fitted with values less than 2.5. Random coil and DNA molecules, as well as aggregates often need higher f/f0 values. If a previous spectrum analysis distribution was loaded, these values will be initialized automatically to the values derived from the loaded distribution. f/f0 resolution: This value controls the number of divisions made in the f/f0 ratio range.

Control Functions:

Help: This help file Load Distribution: The average temperature calculated from the entire run Cancel: Close the control module window. Accept Accept the current settings for the analysis